Identification of unknown Lab Report Example | Topics and Well Written Essays - 1000 words

Identification of unknown - Lab Report Example We were provided with number coded 24- hour broth culture containing a mixture of any two bacteria (gram +ve or gram –ve), 1 EMB plate, 1 blood agar plate, several inoculating loops and TSA slants. Other materials (mediums and reagents) required were requested from the laboratory technician depending on the type of tests that were to be carried out to facilitate in identifying the unknown bacteria. There were several tests to be carried out to identify the unknown bacteria. On receiving the less than 24 hours broth culture which contained two types of bacteria (G +ve and G- ve), I prepared a stock culture for culture purification. Labelled the bottom of blood agar plate (nonselective medium) with my identification codes, then chose one of the unknown culture, opened the petri dish carefully without placing the top of dish down on the bench. I used the inoculating loop to gently swipe across an area of the bacteria to transfer from the dish to the loop and gently made three successive streaks of the bacteria onto the agar surface of the labelled plate. Incubated the plate at 35o C for almost a week, I kept checking it regularly until single colonies had grown. Then I made a subculture of a single colony on TSA slant, incubated it at 35o C after good growth, I removed from the incubator and stored at room temperature. After acquiring the individual colonies, I performed a gram stain test using EMB (eosin Methylene blue) and lactose agar plate. Collected bacterial colony from and streaked on the EMB plate and waited for some days to observe the result. The unknown bacteria was Gram negative. With G –ve result I had to carry more tests: Oxidase test, Lactose fermentation test, Indole test, Bile Esculin test, Motility test, Methyl Red -Voges Proskauer test, and Lysine decarboxylase test. Oxidase test: Obtained a liquid culture stock of my gram

Exercises BYP10-1 and BYP11-10 Essay Example | Topics and Well Written Essays - 1000 words

Exercises BYP10-1 and BYP11-10 - Essay Example ‘Write the press release convincing the stockholders that the stock dividend is just as good as a cash dividend’, he orders. ‘Just watch our stock rise when we announce the stock dividend; it must be a good thing if that happens.’ There is nothing unethical about President Mailor’s actions, as declaring dividends are at the sole discretion of the company. Moreover, Greenwood has paid out cash dividends for over 15 years, when the company was doing better in terms of cash as well as earnings. At the time of crisis, where there is only enough liquid assets to take care of day - to – day operational activities, the decision taken by President Mailor is very reasonable. When stock dividends are declared, the stockholders are given additional stocks based on the number of stocks originally held by them. If this involves, adding new shares to the company, then the total number of stocks are increased; however the value of the stock is reduced, so that the total stockholders’ equity remains the same. Hence a stock dividend increases the number of stocks whereas the total value of stockholder equity is unchanged. As a stockholder, a cash dividend is preferred, as it is paid out immediately and adds value to the stockholder. However a stock dividend is of lesser value to the stockholder, as it just increases the number of stocks held and does not add value immediately. The stock dividend appreciates only when the value of the company goes up in the future. But there is also a possibility of the company’s value going down. Hence a cash dividend is more preferred by the